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rhMPO 
编号: BA1001
规格: 250ug
价格:
2500.00
类型: 2-3 mg/mL
购买数量:
简介

Product Name:Recombinant human myeloperoxidase (rhMPO)

Description:Myeloperoxidase (MPO, E.C. number: 1.11.2.2) belongs to the heme peroxidasefamily. It is highly expressed in leukocytes, neutrophils, and monocytes.MPO playsa crucial role in host defense by generation of potent oxidants. It candestruct various microorganisms including bacteria, fungi, and viruses. The mature myeloperoxidase is a tetramer composed of 2light chains and 2 heavy chains. Molecular weight of the maturemyeloperoxidase is ~150,000 Da. rhMPO is a single chain possessing the sameactivity and enzymatic property as MPO purified from neutrophils. Molecularweight of rhMPO is ~90 kDa.

Entrez Gene ID: 4353

Source:Purified from over-expressing human cells. rhMPO does not contain any tag.

Purity: 95% by SDS-PAGE

Endotoxin levels:<0.3EU by the Limulus Amoebocyte Lysate test

Microbiology:  Negative for bacteria, mycobacteria andfungi.

Form:sterile in PBS

Concentration:2-3 mg/mL

Activity: 7500-8000U/mg protein

Unit definition:  One unit produces an increase of 1.0 per minute at 470 nm atpH 7.0 and 25 °C using guaiacol as substrate.

Storage:Shortterm (< 2 month): at 4°C;long term (> 1 year): -80°C.Avoid repeated freeze/thaw cycles.

For research or further manufacturing use only.

References:

1.Klebanoff,S.J. (2005) J. Leukoc. Biol. 77:598.

2. Heinecke, J.W. et al. (1993) J. Clin. Invest. 91:2866.

3.Hazen, S.L. and J.W. Heinecke (1997) J. Clin. Invest. 99:2075.

4. Podrez, E.A. et al. (1999) J. Clin. Invest.103:1547.

5. Hazen, S.L. et al. (1996) J. Biol. Chem.271:23080.

6.Bergt, C. et al. (2004) Proc. Natl. Acad. Sci. 101:13032.

7.Eiserich, J.P. et al. (2002) Science 296:2391.



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